MUC1 expressing tumor growth was retarded after human mucin 1 (MUC1) plasmid DNA immunization.

ABSTRACT:

Introduction

Naked DNA is one of the attractive tools for vaccination studies. We studied naked DNA vaccination against the human tumor antigen, mucin, which is encoded by the MUC1 gene.

Methods

We constructed the pcDNA3.0-MUC1 (pcDNA-MUC1) plasmid expressing an underglycosylated MUC1 protein. BALB/c mice were immunized intradermally thrice at 2-weeks intervals with pcDNA-MUC1. Two weeks after the last immunization, tumor challenge experiments were performed using either the CT26 or TA3HA tumor cell lines, both of which transduce human MUC1.

Results

Immune cell population monitoring from pcDNA-MUC1-immunized animals indicated that immune cell activation was induced by MUC1-specific immunization. Using intracellular fluorescence activated cell sorting and enzyme

SUBMITTER: Son HY 

PROVIDER: S-EPMC9289905 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature