Exploration of dilated cardiomyopathy for biomarkers and immune microenvironment: evidence from RNA-seq.

ABSTRACT:

Background

The pathogenic mechanism of dilated cardiomyopathy (DCM) remains to be defined. This study aimed to identify hub genes and immune cells that could serve as potential therapeutic targets for DCM.

Methods

We downloaded four datasets from the Gene Expression Omnibus (GEO) database: GSE141910, GSE3585, GSE42955 and GSE79962. Weighted gene coexpression network analysis (WGCNA) and differential expression analysis were performed to identify gene panels related to DCM. Meanwhile, the CIBERSORT algorithm was used to estimate the immune cells in DCM tissues. Multiple machine learning approaches were used to screen the hub genes and immune cells. Finally, the diagnostic value of the hub genes was assessed by receiver operating characteristic (ROC) analysis. An experimental

SUBMITTER: Fang C 

PROVIDER: S-EPMC9290235 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature