Oxidative stress-induced endothelial cells-derived exosomes accelerate skin flap survival through Lnc NEAT1-mediated promotion of endothelial progenitor cell function.

ABSTRACT:

Background

Flap transplantation is commonly used in reconstructive surgery. A prerequisite for skin flap survival is sufficient blood supply. However, such approaches remain unclear. This study aimed to explore the underlying mechanisms of exosomes derived from human umbilical vascular endothelial cells (HUVECs) exposed to oxidative stress on endothelial progenitor cells (EPCs) and their subsequent influence on the survival of skin flaps.

Methods

HUVECs were treated with various concentrations of H₂O₂ to establish an oxidative stress model. To investigate the effects of H₂O₂-HUVEC-Exos and HUVEC-Exos, Cell Counting Kit-8, tube formation, invasion assays, and quantitative real-time polymerase chain reaction (qRT-PCR) were performed

SUBMITTER: Guo L 

PROVIDER: S-EPMC9290268 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature