Low oxygen levels decrease adaptive immune responses and ameliorate experimental asthma in mice.

ABSTRACT:

Background

High-altitude therapy has been used as add-on treatment for allergic asthma with considerable success. However, the underlying mechanisms remain unclear. In order to investigate the possible therapeutic effects of high-altitude therapy on allergic asthma, we utilized a new in vivo mouse model.

Methods

Mice were treated with house dust mite (HDM) extract over 4 weeks and co-exposed to 10% oxygen (Hyp) or room air for the final 2 weeks. Experimental asthma was assessed by airway hyper-responsiveness, mucus hypersecretion and inflammatory cell recruitment. Isolated immune cells from mouse and allergic patients were stimulated in vitro with HDM under Hyp and normoxia in different co-culture systems to analyse the adaptive immune response.

Results

Compared to HD

SUBMITTER: Hochgerner M 

PROVIDER: S-EPMC9290649 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature