Intradermal lipopolysaccharide challenge as an acute in vivo inflammatory model in healthy volunteers.

ABSTRACT:

Aims

Whereas intravenous administration of Toll-like receptor 4 ligand lipopolysaccharide (LPS) to human volunteers is frequently used in clinical pharmacology studies, systemic use of LPS has practical limitations. We aimed to characterize the intradermal LPS response in healthy volunteers, and as such qualify the method as local inflammation model for clinical pharmacology studies.

Methods

Eighteen healthy male volunteers received 2 or 4 intradermal 5 ng LPS injections and 1 saline injection on the forearms. The LPS response was evaluated by noninvasive (perfusion, skin temperature and erythema) and invasive assessments (cellular and cytokine responses) in skin biopsy and blister exudate.

Results

LPS elicited a visible response and returned to baseline at 48 hours.

SUBMITTER: Buters TP 

PROVIDER: S-EPMC9290695 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature