Anti-apoptotic effects of human gingival mesenchymal stromal cells on polymorphonuclear leucocytes.

ABSTRACT:

Objectives

Polymorphonuclear leucocytes (PMNs) constitute the first line of host defence and are crucial in maintaining periodontal health. Their survival and function are modulated by mesenchymal stromal cells (MSCs) from different origin. Gingival MSCs (GMSCs) play an important role in maintaining oral health and in the initial inflammatory response. The present study aimed to investigate the effects of GMSCs on PMNs apoptosis and reactive oxygen species (ROS) production.

Methods

PMNs were either directly incubated with untreated, interleukin (IL)-1β- or tumour necrosis factor (TNF)-α-treated GMSCs or stimulated with their conditioned media. Resulting ROS production was evaluated by dichlorofluorescin diacetate staining, whereas PMNs apoptosis was assessed by Annexin V sta

SUBMITTER: Blufstein A 

PROVIDER: S-EPMC9290793 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature