Validation of inducible basophil biomarkers: Time, temperature and transportation.

ABSTRACT:

Background

The short stability window of several hours from blood collection to measuring basophil activation has limited the use of flow cytometry-based basophil activation assays in clinical settings. We examine if it is possible to extend this window to 1 day allowing for shipment of samples between laboratories. Several options exist for reporting the results including reporting all the measured values directly, calculating ratios and reporting a single value covering all measured results. Each of these options have different stability and value to the physician.

Methods

Whole blood samples from peanut allergic patients were stimulated with four different peanut concentrations at Day 0, Day 1, and Day 2. Samples were stored under temperature-controlled conditions. Flow c

SUBMITTER: Kim T 

PROVIDER: S-EPMC9291082 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature