MiRNA-31 increases radiosensitivity through targeting STK40 in colorectal cancer cells.

ABSTRACT:

Objective

To propose and verify that miRNA-31 increases the radiosensitivity of colorectal cancer and explore its potential mechanism.

Method

A bioinformatics analysis was performed to confirm that the expression of miRNA-31 was higher in colorectal cancer than in normal colorectal tissue. The expression of miRNA-31 was detected to verify the change in its expression in a radiotherapy-resistant cell line. Methylation was detected to explore the cause of the decrease in miRNA-31 expression. Overexpression or inhibition of miRNA-31 further confirmed the change in its expression in colorectal cancer cell lines. Bioinformatics methods were used to screen the downstream target genes and for experimental verification. A luciferase assay was performed to determine the miRNA-31 bind

SUBMITTER: Zhang W 

PROVIDER: S-EPMC9291185 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature