Mitochondrial adaptations to inactivity in diaphragm muscle fibers.

ABSTRACT: Type I and IIa diaphragm muscle (DIAm) fibers comprise slow and fast fatigue-resistant motor units that are recruited to accomplish breathing and thus have a high duty cycle. In contrast, type IIx/IIb fibers comprise more fatigable fast motor units that are infrequently recruited for airway protective and straining behaviors. We hypothesize that mitochondrial structure and function in type I and IIa DIAm fibers adapt in response to inactivity imposed by spinal cord hemisection at C₂ (C₂SH). At 14 days after C₂SH, the effect of inactivity on mitochondrial structure and function was assessed in DIAm fibers. Mitochondria in DIAm fibers were labeled using MitoTracker Green (Thermo Fisher Scientific), imaged in three-dimensions (3-D) by fluorescence confocal mic

SUBMITTER: Brown AD 

PROVIDER: S-EPMC9291409 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature