Design of transfections: Implementation of design of experiments for cell transfection fine tuning.

ABSTRACT: Transfection is the process by which nucleic acids are introduced into eukaryotic cells. This is fundamental in basic research for studying gene function and modulation of gene expression as well as for many bioprocesses in the manufacturing of clinical-grade recombinant biologics from cells. Transfection efficiency is a critical parameter to increase biologics' productivity; the right protocol has to be identified to ensure high transfection efficiency and therefore high product yield. Design of experiments (DoE) is a mathematical method that has become a key tool in bioprocess development. Based on the DoE method, we developed an operational flow that we called "Design of Transfections" (DoT) for specific transfection modeling and identification of the optimal transfection conditions. As

SUBMITTER: Mancinelli S 

PROVIDER: S-EPMC9291525 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature