A high-temperature water vapor equilibration method to determine non-exchangeable hydrogen isotope ratios of sugar, starch and cellulose.

ABSTRACT: The analysis of the non-exchangeable hydrogen isotope ratio (δ² H_ne ) in carbohydrates is mostly limited to the structural component cellulose, while simple high-throughput methods for δ² H_ne values of non-structural carbohydrates (NSC) such as sugar and starch do not yet exist. Here, we tested if the hot vapor equilibration method originally developed for cellulose is applicable for NSC, verified by comparison with the traditional nitration method. We set up a detailed analytical protocol and applied the method to plant extracts of leaves from species with different photosynthetic pathways (i.e., C₃ , C₄ and CAM). δ² H_ne of commercial sugars and starch from different classes and sources, ranging from -

SUBMITTER: Schuler P 

PROVIDER: S-EPMC9291759 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature