Vesicular Acetylcholine Transporter Alters Cholinergic Tone and Synaptic Plasticity in DYT1 Dystonia.

ABSTRACT:

Background

Acetylcholine-mediated transmission plays a central role in the impairment of corticostriatal synaptic activity and plasticity in multiple DYT1 mouse models. However, the nature of such alteration remains unclear.

Objective

The aim of the present work was to characterize the mechanistic basis of cholinergic dysfunction in DYT1 dystonia to identify potential targets for pharmacological intervention.

Methods

We utilized electrophysiology recordings, immunohistochemistry, enzymatic activity assays, and Western blotting techniques to analyze in detail the cholinergic machinery in the dorsal striatum of the Tor1a^+/- mouse model of DYT1 dystonia.

Results

We found a significant increase in the vesicular acetylcholine transporter (VAChT) protein l

SUBMITTER: Tassone A 

PROVIDER: S-EPMC9291835 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature