Screening for bovine papillomavirus type 13 (BPV13) in a European population of sarcoid-bearing equids.

ABSTRACT:

Background

Bovine papillomavirus types 1 and 2 (BPV1 and BPV2) are accepted aetiological agents of equine sarcoids. Recently, genetically similar BPV13 has been identified from equine sarcoids in Brazil.

Objectives

To determine whether BPV13 DNA can be also found in sarcoid-affected horses in Austria, and donkeys in Northern Italy and the UK, and should hence be considered in the context of vaccine-mediated sarcoid prevention.

Study design

Cross sectional study.

Methods

A total of 194 archival, equine and asinine DNA isolates derived from confirmedly delta-BPV-positive tumours were subjected to quality control by photometric analysis and equine beta-actin PCR. Isolates with DNA concentrations >0.9 ng/µl and confirmed PCR-compatibility (n = 135) were subsequentl

SUBMITTER: Jindra C 

PROVIDER: S-EPMC9292424 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature