Haploid, diploid, and pooled exome capture recapitulate features of biology and paralogy in two non-model tree species.

ABSTRACT: Despite their suitability for studying evolution, many conifer species have large and repetitive giga-genomes (16-31 Gbp) that create hurdles to producing high coverage SNP data sets that capture diversity from across the entirety of the genome. Due in part to multiple ancient whole genome duplication events, gene family expansion and subsequent evolution within Pinaceae, false diversity from the misalignment of paralog copies creates further challenges in accurately and reproducibly inferring evolutionary history from sequence data. Here, we leverage the cost-saving benefits of pool-seq and exome-capture to discover SNPs in two conifer species, Douglas-fir (Pseudotsuga menziesii var. menziesii (Mirb.) Franco, Pinaceae) and jack pine (Pinus banksiana Lamb., Pinaceae). We show, using minima

SUBMITTER: Lind BM 

PROVIDER: S-EPMC9292622 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature