Technical considerations in Hi-C scaffolding and evaluation of chromosome-scale genome assemblies.

ABSTRACT: The recent development of ecological studies has been fueled by the introduction of massive information based on chromosome-scale genome sequences, even for species for which genetic linkage is not accessible. This was enabled mainly by the application of Hi-C, a method for genome-wide chromosome conformation capture that was originally developed for investigating the long-range interaction of chromatins. Performing genomic scaffolding using Hi-C data is highly resource-demanding and employs elaborate laboratory steps for sample preparation. It starts with building a primary genome sequence assembly as an input, which is followed by computation for genome scaffolding using Hi-C data, requiring careful validation. This article presents technical considerations for obtaining optimal Hi-C sca

SUBMITTER: Yamaguchi K 

PROVIDER: S-EPMC9292758 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature