Improved high throughput protocol for targeting eukaryotic symbionts in metazoan and eDNA samples.

ABSTRACT: Eukaryote symbionts of animals are major drivers of ecosystems not only because of their diversity and host interactions from variable pathogenicity but also through different key roles such as commensalism and to different types of interdependence. However, molecular investigations of metazoan eukaryomes require minimising coamplification of homologous host genes. In this study we (1) identified a previously published "antimetazoan" reverse primer to theoretically enable amplification of a wider range of microeukaryotic symbionts, including more evolutionarily divergent sequence types, (2) evaluated in silico several antimetazoan primer combinations, and (3) optimised the application of the best performing primer pair for high throughput sequencing (HTS) by comparing one-step and two-step

SUBMITTER: Minardi D 

PROVIDER: S-EPMC9292944 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature