Comparative evaluation of polynomial and Lorentzian lineshape-fitted amine CEST imaging in acute ischemic stroke.

ABSTRACT:

Purpose

Chemical exchange saturation transfer signals from amines are sensitive to pH, and detection of these signals can serve as an alternative pH imaging method to amide proton transfer (APT). However, conflicting results regarding amine CEST imaging at 2 ppm in ischemic stroke have been reported. Here, we correlated amine CEST with APT in animal stroke models to evaluate its specificity to pH, and investigated the reason for the different results through simulations and sample studies.

Methods

A three-point quantification method was used to quantify APT. A polynomial fit method and a multiple-pool Lorentzian fit method were used to quantify amine CEST. Samples of creatine and glutamate were prepared to study the different CEST effects from arginine amine and fast exchang

SUBMITTER: Cui J 

PROVIDER: S-EPMC9293005 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature