Insights into the structural dynamics and helicase-catalyzed unfolding of plant RNA G-quadruplexes.

ABSTRACT: RNA G-quadruplexes (rG4s) are noncanonical RNA secondary structures formed by guanine (G)-rich sequences. These complexes play important regulatory roles in both animals and plants through their structural dynamics and are closely related to human diseases and plant growth, development, and adaption. Thus, studying the structural dynamics of rG4s is fundamentally important; however, their folding pathways and their unfolding by specialized helicases are not well understood. In addition, no plant rG4-specialized helicases have been identified. Here, using single-molecule FRET, we experimentally elucidated for the first time the folding pathway and intermediates, including a G-hairpin and G-triplex. In addition, using proteomics screening and microscale thermophoresis, we identified and vali

SUBMITTER: Wang L 

PROVIDER: S-EPMC9293640 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature