Structure and Function of Redox-Sensitive Superfolder Green Fluorescent Protein Variant.

ABSTRACT: Aims: Genetically encoded green fluorescent protein (GFP)-based redox biosensors are widely used to monitor specific and dynamic redox processes in living cells. Over the last few years, various biosensors for a variety of applications were engineered and enhanced to match the organism and cellular environments, which should be investigated. In this context, the unicellular intraerythrocytic parasite Plasmodium, the causative agent of malaria, represents a challenge, as the small size of the organism results in weak fluorescence signals that complicate precise measurements, especially for cell compartment-specific observations. To address this, we have functionally and structurally characterized an enhanced redox biosensor superfolder roGFP2 (sfroGFP2). Results:<

SUBMITTER: Heimsch KC 

PROVIDER: S-EPMC9293687 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature