A new method for quantitative analysis of M13 bacteriophage by atomic force microscopy

ABSTRACT: Quantitative analysis is essential for virus research, especially in determining the virus titer. The classical method plaque assay is time-consuming, complex, and difficult for the phages that cannot form apparent plaque on the solid medium. In order to realize rapid and effective detection, a new method combining atomic force microscopy (AFM) observation and mathematical calculation is established. In this research, M13 phages with an appropriate dilution ratio were observed and counted by AFM. Based on the counting results, the titer of M13 phages can be calculated simply through mathematical substitution. Instead of cultivating overnight in plaque assay, this new method can be implemented within a few hours. Moreover, it is a method that can achieve visualization for titer determinatio

SUBMITTER: Wu Y 

PROVIDER: S-EPMC9293941 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature