Domain-level epitope mapping of polyclonal antibodies against HER-1 and HER-2 receptors using phage display technology.

ABSTRACT: HER-1 and HER-2 are tumor-associated antigens overexpressed in several epithelial tumors, and successfully targeted by therapeutic approaches against cancer. Vaccination with their recombinant extracellular domains has had encouraging results in the pre-clinical setting. As complex humoral responses targeting multiple epitopes within each antigen are the ultimate goal of such active immunotherapy strategies, molecular dissection of the mixture of antibody specificities is required. The current work exploits phage display of antigenic versions of HER-1 and HER-2 domains to accomplish domain-level epitope mapping. Recognition of domains I, III and IV of both antigens by antibodies of immunized mice was shown, indicating diverse responses covering a broad range of antigenic regions. The combi

SUBMITTER: Perez-Martinez D 

PROVIDER: S-EPMC9293994 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature