Single-cell RNA Sequencing Analysis Reveals New Immune Disorder Complexities in Hypersplenism.

ABSTRACT: Hypersplenism (HS) is a concomitant symptom of liver or blood disease. Not only does the treatment of HS face challenges, but the transcriptome of individual cells is also unknown. Here, the transcriptional profiles of 43,037 cells from four HS tissues and one control tissue were generated by the single-cell RNA sequencing and nine major cell types, including T-cells, B-cells, NK cells, hematopoietic stem cells, neutrophil cells, mast cells, endothelial cells, erythrocytes, and dendritic cells were identified. Strikingly, the main features were the lack of CCL5⁺ B-cells in HS and the presence of SESN1⁺ B cells in HS with hepatocellular carcinoma (HS-HCC). In cell-cell interaction analysis, CD74-COPA and CD94-HLA-E in HS were found to be up-regulated. We

SUBMITTER: Zhao HC 

PROVIDER: S-EPMC9294158 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature