A novel 3D pillar/well array platform using patient-derived head and neck tumor to predict the individual radioresponse.

ABSTRACT: Predicting individual radiotherapy (RT) response is valuable in managing head and neck squamous cell carcinoma (HNSCC). We assessed the feasibility of our novel 3D culture platform to measure radioresponse using patient-derived cells (PDCs) from HNSCC patients. Cells from the FaDu line and tumor samples from 39 HNSCC patients were cultivated serially in Matrigel^TM on a 3D pillar/well array culture system. The 3D tumor models were exposed to 0 to 8 Gy of radiation dose, and the radioresponse index (RT_auc, area under the dose-response curve) was measured quantitatively with Calcein AM staining of live tumor cells. Calcein AM fluorescence showed reduced density and the number of FaDu colonies as radiation increased, implying a dose-dependent effect on cell viability in t

SUBMITTER: Lee DW 

PROVIDER: S-EPMC9294182 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature