A naturally occurring membrane-anchored Gα_s variant, XLα_s, activates phospholipase Cβ4.

ABSTRACT: Extra-large stimulatory Gα (XLα_s) is a large variant of G protein α_s subunit (Gα_s) that uses an alternative promoter and thus differs from Gα_s at the first exon. XLα_s activation by G protein-coupled receptors mediates cAMP generation, similarly to Gα_s; however, Gα_s and XLα_s have been shown to have distinct cellular and physiological functions. For example, previous work suggests that XLα_s can stimulate inositol phosphate production in renal proximal tubules and thereby regulate serum phosphate levels. In this study, we show that XLα_s directly and specifically stimulates a specific isoform of phospholipase Cβ (PLCβ), PLCβ4, both in transfected cells and with purified protein compone

SUBMITTER: Phan HTN 

PROVIDER: S-EPMC9294334 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature