Novel ELISA for the specific detection of protease NEXIN-1 in human biological samples.

ABSTRACT:

Introduction

Serpin E2 or protease nexin-1 (PN-1) is a glycoprotein belonging to the serpin superfamily, whose function is closely linked to its ability to inhibit thrombin and proteases of the plasminergic system.

Objectives

In the absence of specific quantitative methods, an ELISA for the quantification of human PN-1 was characterized and used in biological fluids.

Methods

The ELISA for human PN-1 was developed using two monoclonal antibodies raised against human recombinant PN-1. PN-1 was quantified in plasma, serum, platelet secretion from controls and patients with hemophilia A and in conditioned medium of aortic tissue.

Results

A linear dose-response curve was observed between 2 and 35 ng/mL human PN-1. Intra- and interassay coefficients of variation were

SUBMITTER: Venisse L 

PROVIDER: S-EPMC9294866 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature