Control-Based Continuation: A New Approach to Prototype Synthetic Gene Networks.

ABSTRACT: Control-Based Continuation (CBC) is a general and systematic method to carry out the bifurcation analysis of physical experiments. CBC does not rely on a mathematical model and thus overcomes the uncertainty introduced when identifying bifurcation curves indirectly through modeling and parameter estimation. We demonstrate, in silico, CBC applicability to biochemical processes by tracking the equilibrium curve of a toggle switch, which includes additive process noise and exhibits bistability. We compare the results obtained when CBC uses a model-free and model-based control strategy and show that both can track stable and unstable solutions, revealing bistability. We then demonstrate CBC in conditions more representative of an in vivo experiment using an agent-based simulator

SUBMITTER: de Cesare I 

PROVIDER: S-EPMC9295158 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature