Phagocytosis converts infiltrated monocytes to microglia-like phenotype in experimental brain ischemia.

ABSTRACT:

Background

Monocyte-derived macrophages (MDMs) and microglia elicit neural inflammation and clear debris for subsequent tissue repair and remodeling. The role of infiltrating MDMs in the injured brain, however, has been controversial due to overlapping antigen expression with microglia. In this study, we define the origin and function of MDMs in cerebral ischemia.

Methods

Using adoptive transfer of GFP⁺ splenocytes into adult asplenic mice subjected to transient middle cerebral artery occlusion, we compared the role of CD11b⁺/CD45⁺/NK1.1^-/Ly6G^- MDMs and microglia in the ischemic brain. The phagocytic activities of MDMs and microglia were measured by the uptake of fluorescent beads both in vivo with mice infused with GFP<

SUBMITTER: Ju H 

PROVIDER: S-EPMC9295522 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature