Identification of a Stable Chromosomal Tandem Multicopy of bla_VIM-63, a New bla_VIM-2 Carbapenemase.

ABSTRACT: This study characterizes a new genetic structure containing a multicopy of a bla_VIM-2 variant with an A676C substitution, bla_VIM-63. This gene was detected on the chromosome of two carbapenem-resistant clinical strains of Citrobacter freundii ST22 recovered from two patients, separated by a 6-month period, and previously in Pseudomonas aeruginosa ST2242 from the same hospital unit. Short-read sequencing was used to characterize the new variant in both species, and long-read sequencing was used to characterize the genome of C. freundii. On the P. aeruginosa chromosome, the bla_VIM-63 gene was inserted between ISPsy 42-type sequences, flanked by an intl1 sequence, nearby aph(3')-VI, and sul1. On the C. freundii

SUBMITTER: Pulido MR 

PROVIDER: S-EPMC9295573 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature