The Astrin-SKAP complex reduces friction at the kinetochore-microtubule interface.

ABSTRACT: The kinetochore links chromosomes to spindle microtubules to drive chromosome segregation at cell division. While we know nearly all mammalian kinetochore proteins, how these give rise to the strong yet dynamic microtubule attachments required for function remains poorly understood. Here, we focus on the Astrin-SKAP complex, which localizes to bioriented kinetochores and is essential for chromosome segregation but whose mechanical role is unclear. Live imaging reveals that SKAP depletion dampens the movement and decreases the coordination of metaphase sister kinetochores and increases the tension between them. Using laser ablation to isolate kinetochores bound to polymerizing versus depolymerizing microtubules, we show that without SKAP, kinetochores move slower on both polymerizing and de

SUBMITTER: Rosas-Salvans M 

PROVIDER: S-EPMC9295892 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature