Kinetochore size scales with chromosome size in bimodal karyotypes of Agavoideae.

ABSTRACT:

Background and aims

In eukaryotes, the total kinetochore size (defined as a chromosomal region containing CENH3-positive nucleosomes) per nucleus strongly correlates with genome size, a relationship that has been hypothesized to stem from general intracellular scaling principles. However, if larger chromosomes within a karyotype required larger kinetochores to move properly, it could also be derived from the mechanics of cell division.

Methods

We selected seven species of the plant subfamily Agavoideae whose karyotypes are characterized by the presence of small and very large chromosomes. We visualized the kinetochore regions and chromosomes by immunolabelling with an anti-CENH3 antibody and DAPI (6'-diamidino-2-phenylindole) staining. We then employed 2D widefield and 3D su

SUBMITTER: Plackova K 

PROVIDER: S-EPMC9295917 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature