Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells.

ABSTRACT: Centrosomes are microtubule-organizing centers comprised of a pair of centrioles and the surrounding pericentriolar material. Abnormalities in centriole number are associated with cell division errors and can contribute to diseases such as cancer. Centriole duplication is limited to once per cell cycle and is controlled by the dosage-sensitive Polo-like kinase 4 (PLK4). Here, we show that PLK4 abundance is translationally controlled through conserved upstream open reading frames (uORFs) in the 5' UTR of the mRNA. Plk4 uORFs suppress Plk4 translation and prevent excess protein synthesis. Mice with homozygous knockout of Plk4 uORFs (Plk4 ^Δu/Δu ) are viable but display dramatically reduced fertility because of a significant depletion of pri

SUBMITTER: Phan TP 

PROVIDER: S-EPMC9296005 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature