Transcriptional regulation and chromatin architecture maintenance are decoupled functions at the Sox2 locus.

ABSTRACT: How distal regulatory elements control gene transcription and chromatin topology is not clearly defined, yet these processes are closely linked in lineage specification during development. Through allele-specific genome editing and chromatin interaction analyses of the Sox2 locus in mouse embryonic stem cells, we found a striking disconnection between transcriptional control and chromatin architecture. We traced nearly all Sox2 transcriptional activation to a small number of key transcription factor binding sites, whose deletions have no effect on promoter-enhancer interaction frequencies or topological domain organization. Local chromatin architecture maintenance, including at the topologically associating domain (TAD) boundary downstream from the Sox2 enhancer, is wi

SUBMITTER: Taylor T 

PROVIDER: S-EPMC9296009 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature