Detection of cardiac apoptosis by [¹⁸F]ML-10 in a mouse model of permanent LAD ligation.

ABSTRACT:

Purpose

The loss of viable cardiac cells and cell death by myocardial infarction (MI) is still a significant obstacle in preventing deteriorating heart failure. Imaging of apoptosis, a defined cascade to cell death, could identify areas at risk.

Procedures

Using 2-(5-[¹⁸F]fluoropentyl)-2-methyl-malonic acid ([¹⁸F]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in murine hearts after permanent ligation of the left anterior descending artery (LAD) inducing myocardial infarction (MI). 2-deoxy-2-[¹⁸F]fluoro-D-glucose ([¹⁸F]FDG) PET imaging localized the infarct area after MI. Histology by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining validated apoptosis in the heart.

SUBMITTER: Fischer M 

PROVIDER: S-EPMC9296384 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature