SBSN drives bladder cancer metastasis via EGFR/SRC/STAT3 signalling.

ABSTRACT:

Background

Patients with metastatic bladder cancer have very poor prognosis and predictive biomarkers are urgently needed for early clinical detection and intervention. In this study, we evaluate the effect and mechanism of Suprabasin (SBSN) on bladder cancer metastasis.

Methods

A tissue array was used to detect SBSN expression by immunohistochemistry. A tumour-bearing mouse model was used for metastasis evaluation in vivo. Transwell and wound-healing assays were used for in vitro evaluation of migration and invasion. Comprehensive molecular screening was achieved by western blotting, immunofluorescence, luciferase reporter assay, and ELISA.

Results

SBSN was found markedly overexpressed in bladder cancer, and indicated poor prognosis of patients. SBSN promoted invasio

SUBMITTER: Zhou Z 

PROVIDER: S-EPMC9296541 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature