HDAC4 promotes the growth and metastasis of gastric cancer via autophagic degradation of MEKK3.

ABSTRACT:

Background

Histone deacetylases (HDACs) have been shown to be involved in tumorigenesis, but their precise role and molecular mechanisms in gastric cancer (GC) have not yet been fully elucidated.

Methods

Bioinformatics screening analysis, qRT-PCR, and immunohistochemistry (IHC) were used to identify the expression of HDAC4 in GC. In vitro and in vivo functional assays illustrated the biological function of HDAC4. RNA-seq, GSEA pathway analysis, and western blot revealed that HDAC4 activated p38 MAPK signalling. Immunofluorescence, western blot, and IHC verified the effect of HDAC4 on autophagy. ChIP and dual-luciferase reporter assays demonstrated that the transcriptional regulation mechanism of HDAC4 and ATG4B.

Results

HDAC4 is upregulated in GC and correlates with p

SUBMITTER: Zang WJ 

PROVIDER: S-EPMC9296555 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature