Un-biased housekeeping gene panel selection for high-validity gene expression analysis.

ABSTRACT: Differential gene expression normalised to a single housekeeping (HK) is used to identify disease mechanisms and therapeutic targets. HK gene selection is often arbitrary, potentially introducing systematic error and discordant results. Here we examine these risks in a disease model of brain hypoxia. We first identified the eight most frequently used HK genes through a systematic review. However, we observe that in both ex-vivo and in vivo, their expression levels varied considerably between conditions. When applying these genes to normalise expression levels of the validated stroke target gene, inducible Nox4, we obtained opposing results. As an alternative tool for unbiased HK gene selection, software tools exist but are limited to individual datasets lacking genome-wide search capabilit

SUBMITTER: Casas AI 

PROVIDER: S-EPMC9296577 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature