Discovery of lignin-transforming bacteria and enzymes in thermophilic environments using stable isotope probing.

ABSTRACT: Characterizing microorganisms and enzymes involved in lignin biodegradation in thermal ecosystems can identify thermostable biocatalysts. We integrated stable isotope probing (SIP), genome-resolved metagenomics, and enzyme characterization to investigate the degradation of high-molecular weight, ¹³C-ring-labeled synthetic lignin by microbial communities from moderately thermophilic hot spring sediment (52 °C) and a woody "hog fuel" pile (53 and 62 °C zones). ¹³C-Lignin degradation was monitored using IR-GCMS of ¹³CO₂, and isotopic enrichment of DNA was measured with UHLPC-MS/MS. Assembly of 42 metagenomic libraries (72 Gb) yielded 344 contig bins, from which 125 draft genomes were produced. Fourteen genomes were significantly enriched with ¹

SUBMITTER: Levy-Booth DJ 

PROVIDER: S-EPMC9296663 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature