Rapid characterization of adeno-associated virus (AAV) gene therapy vectors by mass photometry.

ABSTRACT: Recombinant adeno-associated viruses (rAAV) are used extensively as gene delivery vectors in clinical studies, and several rAAV based treatments have already been approved. Significant progress has been made in rAAV manufacturing; however, better and more precise capsid characterization techniques are still needed to guarantee the purity and safety of rAAV preparations. Current analytical techniques used to characterize rAAV preparations are susceptible to background signals, have limited accuracy, or require a large amount of time and material. A recently developed single-molecule technique, mass photometry (MP), measures mass distributions of biomolecules with high-resolution and sensitivity. Here we explore applications of MP for the characterization of capsid fractions. We demonstrate

SUBMITTER: Wu D 

PROVIDER: S-EPMC9296698 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature