Small-scale dosimetry for alpha particle ²⁴¹Am source cell irradiation and estimation of γ-H2AX foci distribution in prostate cancer cell line PC3.

ABSTRACT:

Background

The development of new targeted alpha therapies motivates improving alpha particle dosimetry. For alpha particles, microscopic targets must be considered to estimate dosimetric quantities that can predict the biological response. As double-strand breaks (DSB) on DNA are the main cause of cell death by ionizing radiation, cell nuclei are relevant volumes necessary to consider as targets. Since a large variance is expected of alpha particle hits in individual cell nuclei irradiated by an uncollimated alpha-emitting source, the damage induced should have a similar distribution. The induction of DSB can be measured by immunofluorescent γ-H2AX staining. The cell γ-H2AX foci distribution and alpha particle hits distribution should be comparable and thereby verify the necessity

SUBMITTER: Mellhammar E 

PROVIDER: S-EPMC9296737 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature