Solution structure of a thrombin binding aptamer complex with a non-planar platinum(ii) compound.

ABSTRACT: Thrombin Binding Aptamer (TBA) is a monomolecular well-defined two G-tetrad antiparallel G-quadruplex DNA that inhibits the activity of human α-thrombin. In this report, we synthesized a quasi-cross-shaped platinum(ii) compound (L'₂LPt) with one cyclometalated and two carbene ligands. We found L'₂LPt has selective affinity to bind the TBA G-quadruplex. A fibrinogen clotting assay revealed that L'₂LPt can abrogate the inhibitory activity of TBA against thrombin. We solved the 1 : 1 L'₂LPt-TBA complex structure by NMR, which revealed a unique self-adaptive property of L'₂LPt upon binding to TBA. In the complex, a carbene ligand of L'₂LPt rotates to pair with the cyclometalated ligand to form a plane stacking over half of the T

SUBMITTER: Zhu BC 

PROVIDER: S-EPMC9297526 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature