Sodium butyrate inhibits osteogenesis in human periodontal ligament stem cells by suppressing smad1 expression.

ABSTRACT:

Background

Butyrate is a major subgingival microbial metabolite that is closely related to periodontal disease. It affects the proliferation and differentiation of mesenchymal stem cells. However, the mechanisms by which butyrate affects the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) remain unclear. Here, we investigated the effect of sodium butyrate (NaB) on the osteogenic differentiation of human PDLSCs.

Methods

PDLSCs were isolated from human periodontal ligaments and treated with various concentrations of NaB in vitro. The cell counting kit-8 assay and flow cytometric analysis were used to assess cell viability. The osteogenic differentiation capabilities of PDLSCs were evaluated using the alkaline phosphatase activity assay, alizarin red stai

SUBMITTER: Hou J 

PROVIDER: S-EPMC9297574 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature