QRAT: an R-based stand-alone application for relative expression analysis of RT-qPCR data.

ABSTRACT:

Background

Reverse transcription quantitative real-time PCR (RT-qPCR) is a well-established method for analysing gene expression. Most RT-qPCR experiments in the field of microbiology aim for the detection of transcriptional changes by relative quantification, which means the comparison of the expression level of a specific gene between different samples by the application of a calibration condition and internal reference genes. Due to the numerous data processing procedures and factors that can influence the final result, relative expression analysis and interpretation of RT-qPCR data are still not trivial and often necessitate the use of multiple separate software packages capable of performing specific functions.

Results

Here we present qRAT, a stand-alone desktop applica

SUBMITTER: Flatschacher D 

PROVIDER: S-EPMC9297597 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature