An external quality assessment for the molecular testing of the SARS-CoV-2 virus genome in Zhejiang Province, China.

ABSTRACT: The COVID-19 pandemic has necessitated the rapid expansion of laboratories that conduct SARS-CoV-2 tests. A provincial external quality assessment (EQA) scheme on SARS-CoV-2 tests was organized by Zhejiang Provincial CDC to assess the accuracy of the tests in individual CDC municipal and county laboratories in Zhejiang Province, China. Three positive samples in high, medium, and low concentrations, respectively, were prepared using the serial dilutions from the culture with the viral titer concentration of 1×10^6.3 TCID50/mL, and one negative sample were included. A total of 93 laboratories participated, contributing results from 36 distinct combinations of nucleic acid extraction methods and PCR reagents. There was 100% concordance among all laboratories for all EQA samples, and

SUBMITTER: Pan J 

PROVIDER: S-EPMC9297680 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature