MGMT inactivation as a new biomarker in patients with advanced biliary tract cancers.

ABSTRACT: Biliary tract cancers (BTCs) have poor prognosis and limited therapeutic options. The impact of O⁶ -methylguanine-DNA methyltransferase (MGMT) inactivation in advanced BTC patients is not established. We investigated the prevalence, prognostic, and predictive impact of MGMT inactivation in two multicenter cohorts. MGMT inactivation was assessed through PCR and immunohistochemistry (IHC) in an Italian cohort; the results were then externally validated using RNA sequencing (RNA-seq) data from the BTC subcohort of the Molecularly Aided Stratification for Tumor Eradication Research (MASTER) precision oncology program of the National Center for Tumor Diseases Heidelberg and the German Cancer Consortium. Among 164 Italian cases, 18% presented MGMT promoter hypermethylation (> 14%) and

SUBMITTER: Niger M 

PROVIDER: S-EPMC9297767 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature