Characterization of Brucella spp. and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda.

ABSTRACT:

Background

Abortions cause tremendous economic losses in food-producing animals and may lead to food insecurity.

Objectives

This study aimed to characterize Brucella spp. and other abortigenic pathogens from aborted tissues of cattle.

Methods

For cattle, aborted tissues (n = 19) were cultured, and Brucella spp. were detected using the genus-specific 16S-23S ribosomal DNA interspacer region (ITS) assay and speciated using Brucella abortus, Brucella melitensis, Brucella ovis, and Brucella suis (AMOS) and Bruce-ladder PCR assays. Brucella negative samples were screened using the eight abortigenic pathogens PCR panel. Samples from an abortion outbreak that occurred within a goat tribe were included in this investigation. Sera of females (n = 8) and males (n = 2) were anal

SUBMITTER: Ntivuguruzwa JB 

PROVIDER: S-EPMC9297783 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature