Performance of the StaphGold ELISA test in determining subclinical Staphylococcus aureus infections in dairy cows using a Gaussian mixture model.

ABSTRACT:

Background

A novel ELISA test has been developed to detect antigen-specific IgG in early and late lactation cows in New Zealand.

Objectives

This study was to evaluate the discriminatory ability of the ELISA based on the detection of S. aureus-specific IgG as a screening test.

Methods

The ELISA was used for the composite milk samples taken during routine herd testing in 2018-2019 milking season in New Zealand. In the absence of a gold standard test, the diagnostic specificity and sensitivity was estimated using a Gaussian mixture model.

Results

The ELISA test had a high accuracy (AUC = 0.98) to detect antigen-specific IgG in early and late lactation cows with high somatic cell count due to either subsequent to or contemporaneous with the S. aureus invasion. Usin

SUBMITTER: Yang DA 

PROVIDER: S-EPMC9297801 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature