Development of a Rapid Live SARS-CoV-2 Neutralization Assay Based on a qPCR Readout.

ABSTRACT: Measuring SARS-CoV-2 neutralizing antibodies after vaccination or natural infection remains a priority in the ongoing COVID-19 pandemic to determine immunity, especially against newly emerging variants. The gold standard for assessing antibody-mediated immunity against SARS-CoV-2 are cell-based live virus neutralization assays. These assays usually take several days, thereby limiting test capacities and the availability of rapid results. In this study, therefore, we developed a faster live virus assay, which detects neutralizing antibodies through the early measurement of antibody-mediated intracellular virus reduction by SARS-CoV-2 qRT-PCR. In our assay, Vero E6 cells are infected with virus isolates preincubated with patient sera and controls. After 24 h, the intracellular viral load is

SUBMITTER: Lichtenegger S 

PROVIDER: S-EPMC9297810 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature