A comparative study of mesenchymal stem cells cultured as cell-only aggregates and in encapsulated hydrogels.

ABSTRACT: There is increasing evidence that cells cultured in three-dimensional (3D) settings have superior performance compared to their traditional counterparts in monolayers. This has been attributed to cell-cell and cell-extracellular matrix interactions that more closely resemble the in vivo tissue architecture. The rapid adoption of 3D cell culture systems as experimental tools for diverse applications has not always been matched by an improved understanding of cell behavior in different 3D environments. Here, we studied human mesenchymal stem/stromal cells (hMSCs) as scaffold-free self-assembled aggregates of low and high cell number and compared them to cell-laden alginate hydrogels with and without arginine-glycine-aspartic acid peptides. We observed a significant decrease in the size of ce

SUBMITTER: Passanha FR 

PROVIDER: S-EPMC9297862 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature