Evaluation of inhibitor activity of bacterial sialidase from Clostridium perfringens against Newcastle disease virus in the cell culture model using chicken embryo fibroblast.

ABSTRACT:

Objective

The Newcastle disease virus (NDV) is an infectious disease that causes very high economic losses due to decreased livestock production and poultry deaths. The vaccine's ineffectiveness due to mutation of the genetic structure of the virus impacts obstacles in controlling the disease, especially in some endemic areas. This study aimed to provide an alternative treatment for NDV infection by observing the viral replication inhibitor activity of Clostridium perfringens sialidase in primary chicken embryo fibroblast (CEF) cells.

Materials and methods

The virus was adapted in CEF monolayer cells, then collected thrice using the freeze-thaw method and stored at -20°C for the next step in the challenge procedure. C. perfringens crude sialidase was obtained,

SUBMITTER: Kurnia RS 

PROVIDER: S-EPMC9298094 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature