Profound differences in IgE and IgG recognition of micro-arrayed allergens in hyper-IgE syndromes.

ABSTRACT:

Background

The specificities of IgE and IgG for allergen molecules in patients with inborn errors of immunity (IEI) have not been investigated in detail.

Objective

To study IgE and IgG antibody specificities in patients with defined hyper-IgE syndromes (HIES) using a comprehensive panel of allergen molecules.

Methods

We used chips containing micro-arrayed allergen molecules to analyze allergen-specific IgE and IgG levels in sera from two groups of HIES patients: Autosomal recessive mutations in phosphoglucomutase-3 (PGM3); Autosomal dominant negative mutations of STAT3 (STAT3); and age-matched subjects with allergic sensitizations. Assays with rat basophil leukemia cells transfected with human FcεRI were performed to study the biological relevance of IgE sensitization

SUBMITTER: Garib V 

PROVIDER: S-EPMC9298271 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature